Coding

Part:BBa_K1705008

Designed by: Nathan Hunt   Group: iGEM15_MIT   (2015-09-18)

LuxI

LuxI is an AHL synthetase. The specific AHL produced by this gene product is 3OC6HSL. It is used in quorum sensing systems: one organism expresses LuxI, whose AHL's diffuse freely into the surrounding environment. When an AHL enters a strain expressing LuxR, they bind, and LuxR-AHL activates pLux (there is also a LuxR-repressed version of pLux). The expression of a gene can thus be tied to the surrounding concentration of other (LuxI-expressing) organisms.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 593


[edit]
Categories
Parameters
None